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Blood film

Blood Film Inhaltsangabe & Details

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blood film

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Blood Film Video

Part 2 - Examination of Peripheral Blood Smear

Blood Film Account Options

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When a significant number of abnormal cells are present, they can indicate disease and prompt the doctor to do further testing.

The blood film is primarily used when a FBC with differential, performed with an automated blood cell counter, shows the presence of abnormal or immature cells.

It may also be used when a doctor suspects a deficiency, disease or disorder that is affecting blood cell production, such as an anaemia , decreased or abnormal production of cells in the bone marrow , or increased cell destruction.

A blood film may also be requested when a patient is being treated or monitored for a blood cell-related disease. Findings from the blood film test do not always give a diagnosis but can provide information indicating the presence of an underlying condition and its severity and the need for further diagnostic testing.

Blood film findings may include:. Unlike most other cells, they do not have a nucleus. They are round and flattened like a doughnut but with a depression in the middle instead of a hole biconcave.

With routine staining, due to the haemoglobin inside the RBCs, they appear pink to red in colour with a pale centre.

While not every RBC will be perfect, the presence of many cells that are different in shape or size may indicate a more severe problem.

There may be one or more irregularities present and may include:. All WBCs come from bone marrow stem cells.

In the marrow, they change into two groups: myeloid and lymphoid cells. They then mature into five different types of WBCs. Platelets These are cell fragments that come from large bone marrow cells called megakaryocytes.

Upon release from the bone marrow, they appear as fragments in the peripheral blood. When there is blood vessel injury or other bleeding, the platelets become activated and begin to clump together to form aggregates.

This is the first step in making a blood clot. You must have a sufficient number of platelets to control bleeding. If there are too few, the ability to form a clot is impaired and can be life-threatening.

In some people, too many platelets may be produced, which interferes with the flow of blood and increases a person's risk of developing a blood clot.

These same people may also experience bleeding because many of the extra platelets may be dysfunctional even though they appear normal. Details of platelet number and size is usually part of a FBC.

An abnormally low number or high number of platelets may be further evaluated by preparing a peripheral blood film to visualise any anomalies in shape or size directly.

Some examples of situations or conditions that may affect or invalidate results of a blood film include:.

Automated blood cell counters are used for routine testing of FBCs. These machines provide information based on the shape, size, and electrical or photometric properties RBCs, WBCs and platelets.

A variety of physiological and external stimuli can lead to some variation in the numbers of cells the body produces and the proportions of the different cell.

Automated instruments can often identify the presence of abnormal cells but lack the sophistication to subclassify them definitively.

Cell fragments and platelet clumps, particularly if they are large in size, can be mistakenly counted as WBCs, thus falsely elevating a white cell count.

Medical scientists and haematologists have been trained to identify and classify these abnormalities by examining a blood film.

Neutrophils also called segmented neutrophils, polymorphonuclear cells, polys or PMNs are about 12 microns in diameter and their function is to engulf and destroy invading organisms.

This WBC may be seen in greater numbers during infections, malignancies or extreme stress situations. Using a standard Romanowsky dye, the blood slides are stained to visualise the various cell types better.

The cytoplasm of neutrophils is pale and often contains small pink to purple granules. These granules specific granules and azurophilic granules contain enzymes and proteins that neutralise or destroy microorganisms.

Anomalies of neutrophils include:. They are most often involved in allergic responses and parasitic infections.

Basophils have a multi-lobed nucleus and have many dark blue granules which contain histamine in their cytoplasm. A slight elevation in number may be seen during an allergic response, ulcerative colitis, chronic sinusitis, chicken pox and immunisations.

A significant increase is not uncommon in certain myeloid leukaemias. The nucleus is generally large in relation to the amount of cytoplasm.

The cytoplasm is pale blue and generally does not contain any granules. The nucleus of most lymphocytes is smooth in appearance and is dark blue.

There are two major types of lymphocytes, B cell and T-cell, but they cannot be distinguished when viewed under the microscope using standard staining techniques.

B cells can be differentiated from T cells using specific fluorescent-labelled antibody stains in conjunction with a special instrument called a flow cytometer.

B cells create specific antibodies while T-cells can activate B cells as well as recognise and destroy invading organisms.

They are characterised by their abundant blue-grey cytoplasm that is irregular in shape and have a folded nucleus.

The main function of monocytes is to ingest microorganisms and respond to infection and inflammation by releasing certain proteins monokines that can inactivate bacteria.

When stimulated by cytokines, monocytes can move out of the bloodstream and become tissue macrophages. Blood Film. Send Us Your Feedback.

Choose Topic At a Glance What is being tested? Also Known As. Formal Name. This article was last reviewed on 21 March This article was last modified on 28 January At a Glance.

Why Get Tested? When To Get Tested? Sample Required? Test Preparation Needed? No test preparation is needed. Looking for Test Results?

Looking for Reference Ranges? What is being tested? How is the sample collected for testing? Is any test preparation needed to ensure the quality of the sample?

See More. See Less. Accordion Title. How is it used? When is it requested? What does the test result mean? There may be one or more irregularities present and may include: Anisocytosis - variable sizes of red cells.

Poikilocytosis - various shapes of red cells. These may include echinocytes, acanthocytes, elliptocytes, keratocytes, sickle cells, target cells, teardrop cells dacrocytes , smear cells also known as smudge or basket cells , and schistocytes.

Granulocytes — the three types of cell making up this group are distinguished by granules of characteristic size and colour in their cytoplasm.

Neutrophils - these cells have nuclei with multiple lobes and have pink or purple granules in their cytoplasm. They compose the majority of WBCs in a healthy adult.

They increase in episodes of inflammation, such as with bacterial infections or in rheumatoid arthritis.

Like eosinophils they usually have bi-lobed nuclei. Increased numbers of basophils are not often encountered but may be found with certain haematological diseases most notably some leukaemias and myeloproliferative disorders.

They can ingest particles such as cellular debris, bacteria, or other insoluble particles. Lymphocytes are responsible for the production of antibodies immunoglobulins or have complicated roles in organising the responses of other white blood cells or in recognising and attacking germs or even cancer cells See the section below for more Details on White Blood Cells.

Is there anything else I should know? Some examples of situations or conditions that may affect or invalidate results of a blood film include: The patient has received a recent blood transfusion The patient has increased levels of protein The blood specimen has a blood clot in it Blood collected in the wrong specimen tube Not enough blood collected into the specimen tube Specimen tube not kept at the correct temperature Blood film not prepared or stained correctly Sample too old for preparation of film.

Why hasn't the automated blood cell counter totally replaced the blood film? Vigilante Justice. Use the HTML below. You must be a registered user to use the IMDb rating plugin.

Edit Cast Cast overview, first billed only: Paul Bettany Joe Fairburn Stephen Graham Chrissie Fairburn Brian Cox Lenny Fairburn Mark Strong Robert Seymour Ben Crompton Jason Buliegh Naomi Battrick Jemma Venn Natasha Little Lily Fairburn Sandra Voe Sandra Buliegh Jasper Britton Daniel Heston Adrian Edmondson Tom Tiernan Danny McEvoy Sammy Lucy Lowe Dominic Stuart McQuarrie Learn more More Like This.

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Edit Did You Know? Quotes Robert Seymour : I wouldn't wish that on anyone. Getting away with it. Was this review helpful to you?

Yes No Report this. Add the first question. Edit Details Country: UK. Language: English. Runtime: 92 min.

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Blood Film - Inhaltsverzeichnis

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